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. 2013 Jun;5(3):251–257. doi: 10.3978/j.issn.2072-1439.2013.05.08

Figure 2.

Figure 2

Fluorescent quantitative PCR amplification curve for the standard and test samples and the standard curve of MTB DNA. A. Fluorescent quantitative PCR amplification curve: 1, standard sample, 1×107 copies/mL; 2, standard sample, 1×106 copies/mL; 3, standard sample, 1×105 copies/mL; 4, standard sample, 1×104 copies/mL; 5, standard sample, 1×103 copies/mL; 6, test sample of tuberculosis; 7, H2O; B. Standard curve of MTB DNA: Std, positive standard samples; unknown, test sample; C. Electrophoresis of fluorescent quantitative PCR amplification products: M, DNA marker (DL2000); 1-8, plasma/serum samples from tuberculosis patient 1-8; P, positive control; N, negative control; D. Sequence figure of MTB DNA amplification product; E. DNA sequence of MTB DNA amplification product.