Table 2. Comparison of MTB DNA and plasmid DNA isolated using the four extraction methods.
| Qiagen |
Magnetic bead |
Phenol-chloroform |
Omega |
|||||
|---|---|---|---|---|---|---|---|---|
| MTB | Plasmid | MTB | Plasmid | MTB | Plasmid | MTB | Plasmid | |
| Concentration of experimental sample (mean ± SD)a | 1197.8±648.5 | 423.6±183.4 | 1483.1±457.5 | 714.2±214.3 | 704.5±505.3 | 310±208.8 | 946.7±246.0 | 316.7±135.8 |
| Extraction efficiency (mean ± SD) | 42.7±23.1% | 41.0 ±17.7% | 52.8±16.3% | 69.2±20.7% | 25.1±18.0% | 30.0±20.2% | 33.7±8.8% | 30.7±12.7% |
| Concentration of control sample (mean ± SD)a | 3228.4±2383.3 | 1326.9±1668.9 | 2451.6±1509.1 | 1047.9±539.6 | 2399.1±709.0 | 806.6±249.7 | 4199.5±1983.5 | 1686.4±4681.2 |
| Loss ratio (mean ± SD) | 63.2±19.8% | 68.1±13.8% | 40.1±18.5% | 31.8±20.4% | 70.6±21.0% | 61.6±25.9% | 66.3±8.8% | 81.2±8.1% |
| Inhibition ratio | –15.0% | –28.5% | 12.7% | –1.5% | 14.5% | 21.9% | –49.7% | –63.3% |
| Coefficient of variance | 46.9% | 37.5% | 26.7% | 26.0% | 62.2% | 58.4% | 22.5% | 37.2% |
a: ng/mL.