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. 1982 May;2(5):593–597. doi: 10.1128/mcb.2.5.593

Comparison of phenotypic expression with genotypic transformation by using cloned, selectable markers.

P S Linsley, L Siminovitch
PMCID: PMC369830  PMID: 6287241

Abstract

The frequency of phenotypic expression of the herpes simplex virus type 1 tk and Escherichia coli gpt genes was compared with the frequency of genotypic transformation after calcium phosphate-mediated DNA transfection of a number of tk- and hprt- cell lines. In three of the five lines tested, the frequency of phenotypic expression was at most 10-fold higher than that of genotypic transformation as indicated by frequency of HAT resistance. The remaining two lines showed phenotypic responses which were 50- to 100-fold greater than the genotypic responses. The data indicate that the efficiency of DNA-mediated transformation with some cell lines can be limited by events after the uptake and expression of transfected DNA.

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Selected References

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