Table 2. Pneumococcal isolation-based serotyping results in children (n = 237) with corresponding cmPCR results.
| Serotypes detected by culture (corresponding sts co-detected by cmPCR) |
No. Isolation/Quellung-positive (percentage of specimens) |
No. cmPCR-positive (percentage of specimens)/ number corresponding to positive Quellung (percentage of Quellung-positive specimens) |
Ratio of cmPCR-positives/ culture Quellung-positive |
|---|---|---|---|
| 19F (19F) | 30a (12.2) | 37 (15.7)/30 (100) | 1.2 |
| 23F (23F) | 19 (8.0) | 31 (13.1)/17 (89.5) | 1.6 |
| 6A (6A/6B) | 15a (6.4) | 42 (17.7)/27 (96.4) | 1.5 |
| 6B (6A/6B) | 13 (5.5) | ||
| 14 (14) | 11 (4.6) | 28 (11.8)/11 (100) | 2.5 |
| 11A (11A/11D) | 9a (3.8) | 13 (5.5)/9 (100)a | 1.4 |
| 19B (not in cmPCR assay) | 7 (3.0) | 0 | 0 |
| 1 (1) | 6a (2.5) | 9 (3.8)/6 (100) | 1.5 |
| 13 (13) | 6 (2.5) | 8 (3.4)/4 (66.7) | 1.3 |
| 20 (20) | 5 (2.1) | 7 (3.0)/5 (100)a | 1.4 |
| 15B (15B/15C) | 5 (2.1) | 12 (5.1)/10 (100)b | 1.2 |
| 15C (15B/15C) | 5a (2.1) | ||
| 34 (34) | 5a (2.1) | 9 (3.8)/5 (100) | 1.8 |
| 10A (10A) | 4 (1.7) | 6 (2.5)/4 (100)b | 1.5 |
| 23B (23B) | 4 (1.7) | 7 (3.0)/4 (100) | 1.8 |
| 35B (35B) | 4 (1.7) | 8 (3.4)/4 (100) | 2.0 |
| 3 (3) | 3a (1.3) | 6 (2.5)/2 (66.7) | 2.0 |
| 18A (18A/18B/18C/18D) | 3 (1.3) | 8 (3.4)/4 (100) | 1.8 |
| 18C (18A/18B/18C/18D) | 1 (0.4) | ||
| 17F (17F) | 3 (1.3) | 4 (1.7)/3 (100)b | 1.3 |
| 23A (23A) | 3a (1.3) | 3 (1.3)/3 (100)a | 1.0 |
| 16F (16F) | 3 (1.3) | 8 (3.4)/3 (100) | 2.7 |
| 15A (15A/15F) | 3 (1.3) | 11 (4.6)/3 (100)a | 3.7 |
| 4 (4) | 3 (1.3) | 8 (3.4)/3 (100) | 2.7 |
| 5 (5) | 2 (0.8) | 2 (0.8)/ 2 (100) | 1.0 |
| 7F (7F/7A) | 2 (0.8) | 5 (2.1)/2 (100) | 2.5 |
| 9V (9V/9A) | 2 (0.8) | 2 (0.8)/2 (100) | 1.0 |
| 12F (12F/12A/44/46) | 2 (0.8) | 13 (5.5)/4 (100) | 3.2 |
| 46 (12F/12A/44/46) | 2 (0.8) | ||
| 38 (38/25F/25A) | 2 (0.8) | 9 (3.8)/2 (66.7)a | 3.0 |
| 25A (38/25F/25A) | 1 (0.4) | ||
| 21 (21) | 2a (0.8) | 6 (2.5)/ 2 (100) | 3.0 |
| 6C (6C/6D) | 1 (0.4) | 1 (0.4)/1 (100) | 1.0 |
| 9L (9N/9L) | 1 (0.4) | 1 (0.4)/1 (100) | 1.0 |
| 10F (10F/10C/33C) | 1 (0.4) | 13 (5.5)/1 (100) | 13.0 |
| 19A (19A) | 1 (0.4) | 8 (3.4)/1 (100) | 8.0 |
| 24B (24A/24B/24F) | 1 (0.4) | 7 (3.0)/1 (100) | 7.0 |
| 28F (not in mPCR assay) | 1 (0.4) | 0 | 0 |
| 35A (35A/35C/42) | 1 (0.4) | 2 (0.8)/1 (100) | 2.0 |
| 35F (35F/47F) | 1 (0.4) | 5 (2.1)/1 (100) | 5.0 |
| (2) | 0 | 0/1 (0) | 0 |
| nontypeable (PCR-NT) | 9a (3.8) | 16c (6.8) | 1.8 |
Notes.
a
Found in combination with 1 other serotype in 1–3 specimens.
b
1–3 culture-positive specimen(s) initially found cmPCR-negative for the corresponding serotype were retested using monoplex PCR reaction and were subsequently found positive.
c
Specimens positive for cpsA positive control and negative for serotype or serogroup.