Figure 4.

(A) Group mean extracellular acidification (ECAR) in mpH/min of rhabdomyosarcoma cells treated with either ethanol control (final concentration 0.1%), DNP at 500 or 250 μM, or caffeine at 500 or 250 μM for 24 hours. (B) Basal ECAR of cells treated as described above for 24 hours. (C) Maximum ECAR of cells treated as described above for 24 hours following addition of oligomycin. (D) Maximum ECAR of cells treated as described above for 24 hours following with FCCP in addition to oligomycin.

Notes: * indicates P < 0.05, ** indicates P < 0.01, and *** indicates P < 0.001 compared with control.