Figure 1. A high-throughput assay to monitor Drosophila larval feeding.

(a) Assay schematic. (b) Representative picture of the bottom of a single well of a 96-well plate with larvae treated as in (a). Scale bars: 250 μm. (c) Relative fluorescence of larvae incubated at either 25°C or 4°C during the fluorescein feeding stage (n = 32). Fluorescence normalized to 25°C. Data were compared using Mann Whitney test. (d) Relative fluorescence of larvae that were pre-fed either complete liquid food or liquid food lacking yeast extract overnight (n = 16). Fluorescence plotted relative to animals fed liquid food. Data were compared using t test. (e) Relative fluorescence of larvae of different genotypes: w¹¹¹⁸, Canton-S (CS), Oregon-R (OR), klumpfuss⁰⁹⁰³⁶ (klu) (n = 22–24). Fluorescence plotted relative to w¹¹¹⁸. (f) Relative fluorescence of larvae that were fed liquid food or liquid food supplemented with 400 mM alanine or lysine. Fluorescence plotted relative to liquid food (n = 12). In (e–f), data was compared with Kruskal-Wallis test, followed by Dunn's test. In (c–f), error bars indicate s.e.m. In (c–d) *** p < 0.001. Significant differences are labeled with different letters in (e–f).