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. 2013 Jul 1;210(7):1481–1492. doi: 10.1084/jem.20121523

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© 2013 Kodgire et al.

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Figure 7. — Sodium bisulfite analysis to determine the presence of single-stranded DNA patches. (A) The DT40 cells with either the active or inactivated terminator were fixed and treated with sodium bisulfite (Parsa et al., 2012). The regions upstream and downstream of the poly(A) site of the terminator were amplified, sequenced, and analyzed for single-strand patches with at least 2 consecutive Cs converted to Ts or two consecutive Gs converted to As (minimum strand length). The DNA clone # is shown; the total number of DNA clones analyzed for each region are shown in parentheses. The control DT40 cells that were fixed but not treated with sodium bisulfite did not show any single-stranded DNA patches in any of the four regions. (B) The significant differences (or lack of) are indicated between the respective regions of the active and inactivated terminators.