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. 2013 Jul 1;210(7):1419–1431. doi: 10.1084/jem.20122239

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© 2013 Johnson et al.

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Figure 4. — Shp1-deficient T cells exhibit normal responses to TCR stimulation. (A) T cells were isolated from spleens, labeled with CFSE, and cultured on a 96-well plate coated with cross-linked antibodies against CD3 ± CD28. Cells were harvested 3 d later, stained for CD4 and CD8, and analyzed by flow cytometry. (B) Splenic T cells were sorted into CD44^hi and CD44^lo populations by FACS, labeled with CFSE, and cultured on a 96-well plate coated with cross-linked antibodies against CD3 and CD28 at the indicated concentrations. Cells were analyzed as in A. (C and D) Cells were treated as in A, harvested 6 h after stimulation, and analyzed for IL-2 (C) and TNF (D) expression by intracellular staining; n = 2. Data are representative of three independent experiments. Values for C and D are displayed as ± standard error.

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