Figure 6.

Therapeutic administration of GSIs prolongs survival of AA mice. (A) Representative hematoxylin and eosin staining of BM from one AA mouse each whose treatment with vehicle only (DMSO; top) or GSIs (bottom) was begun 5 d after BMF induction. Bars, 200 µm. (B–E) BM cellularity (B), weight change (C), and circulating white (D) and red cells (E) were determined in vehicle-only–treated AA mice (n = 5) and GSI-treated (i.p.) AA mice (n = 5) beginning 5 d after disease induction. (F) Percentages of BM-infiltrating T cells were determined for control (γIR + DMSO [n = 4] and γIR + GSI [n = 4]), vehicle-treated (BMF + DMSO; n = 5), and GSI-treated (BMF + GSI; n = 5) AA mice. (G) Kaplan–Meier survival estimates for AA mice fed control chow or GSI chow beginning 5 d after disease induction (P = 0.002, log-rank test). Data represent the mean ± SEM (n = 4 to 8 mice/group). **, P < 0.01; ***, P < 0.001; unpaired Student’s t test.