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. 2013 Jul 1;210(7):1463–1479. doi: 10.1084/jem.20112495

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© 2013 Iborra et al.

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Figure 8. — Involvement of PI3Kγ and AKT but not of ERK in the induction of Eomes after antigen stimulation. Purified N-ras^+/+ OT-I CD8⁺ T cells were pretreated or not with the indicated inhibitors for ERK kinase (PD98059) and PI3K (wortmannin), stimulated with 10⁻⁹ M OVA peptide as in Fig. 7 D, and incubated in the presence of the inhibitors for the indicated times (A), or similarly stimulated and treated with the inhibitors for ERK kinase (UO126), PI3K (LY294002), PI3Kα (inhibitor VII) PI3Kβ (VI), PI3Kγ, or AKT (VIII) at graded concentrations, and stained for Eomes after 48 h (B). Concentrations in B are indicated in Materials and methods and the highest to lowest depicted in black to gray to white bars, respectively. Statistical significance is indicated for untreated versus wortmannin treated cells in A (three experiments) and for untreated versus the different treatments in B (two experiments). Results are expressed as mean ± SEM. *, P < 0.05; **, P < 0.005; ***, P < 0.0005.