Figure 2.

Upregulation of RAGE mediates the exacerbated Aβ-AGE toxicity in hippocampal neurons. (a–c) The hippocampal neurons cultured 8 DIV were treated with Aβ or Aβ-AGE for 24 h, then the level of RAGE was analyzed by western blot and immunofluorescence staining. (d–i) The hippocampal neurons cultured 8 DIV were pre-incubated with or without RAGE antibody (Ab) before treatment of Aβ or Aβ-AGE for 24 h, then the viability (d), the apoptotic rate (e), levels of the phosphorylated tau (f and g), and synaptic proteins (h, i) were measured. The level of RAGE and synaptic proteins was normalized to DM1A, and phosphorylated tau was normalized to Tau-5. n=3, *P<0.01 versus Aβ group; ^#P<0.01 versus Aβ-AGE group. Scale bar in c, 50 μm