Figure 4.

Aβ is glycated with an age-dependent increase of AGE in the brains of Tg2576 mice. (a and b) The hippocampal extracts from Tg2576 (Tg) or wild-type (WT) mice at 1, 3, 6, 9, and 12 months were analyzed by dot blot using anti-AGE antibody normalized against DM1A (b). (c and d) The hippocampal extracts from 9-month-old Tg mice were precipitated with AGE or Aβ or IgG antibody, and then the level of Aβ or AGE in the precipitate was measured by western blot using anti-Aβ (c) or anti-AGE (d) antibody. n=5, *P<0.01 versus WT group; ^#P<0.01 versus 6 months in Tg group