Figure 5.

Subcutaneous injection of AG prevents formation of Aβ-AGE in Tg2576 mice. Tg2576 (Tg) mice and the wild-type (WT) littermates at 6-month old were subcutaneously injected with AG (blocker of AGE formation) or NS for 3 months. (a) Level of AGE in the cortex extracts was measured by dot blot and quantitated by normalization against DM1A. **P<0.01 versus WT+NS group; ^##P<0.01 versus Tg+NS group. (b and c) The levels of Aβ1-40 and Aβ1-42 in cortex and hippocampus were estimated by ELISA. hippo: hippocampus. *P<0.05, **P<0.01 versus Tg+NS group. (d–g) The cortex extracts were immunoprecipitated with IgG or AGE or Aβ antibody, and then the levels of Aβ-AGE or AGEs in precipitate were measured by western blot using anti-Aβ or anti-AGE antibody as indicated. **P<0.01 versus Aβ-Tg+NS (e) or AGE-Tg+NS group (g)