Figure 3.

RanBP9 overexpression in APΔE9 mice does not alter spinophilin protein levels in the cerebellum at 12 months of age. (a) Brain homogenates from APΔE9 double-transgenic, APΔE9/RanBP9 triple-transgenic and age-matched WT control mice were subjected to SDS-PAGE electrophoresis and probed with anti-spinophilin antibody to detect spinophilin protein. Immunoblotting using RanBP9-specific monoclonal antibody detected both flag-tagged exogenous RanBP9 and endogenous RanBP9 in the triple-transgenic mice, while WT mice and APΔE9 mice showed expression of endogenous RanBP9 only (panel 2). Flag antibody detected expression of flag-tagged exogenous RanBP9 in APΔE9/RanBP9 triple transgenic mice only (panel 3). (b) Image J quantification did not reveal significant changes in the levels of spinophilin in any of the genotypes. (c) Relative expression levels of endogenous and flag-tagged exogenous RanBP9 levels in the cerebellum of triple-transgenic mice. ANOVA did not reveal significant differences. The data are mean±S.E.M., n=5 for WT and APΔE9 and n=4 for APΔE9/RanBP9 genotypes