Figure 8.

RanBP9 protein levels are inversely correlated to spinophilin levels in the synaptosomes of AD brains. (a) Synatosomes from hippocampus of AD with varying severity of the disease starting from Braak stage 2 (Br-2) to Braak stage 6 (Br-6) classified based on the appearance of neurofibrillary tangles and age-matched control brains were prepared as detailed in the legend to figure 5. Equal amounts of total proteins were subjected to western blots to detect spinophilin, RanBP9-FL, RanBP9 N60 and actin as a loading control using specific antibodies. (b) Quantitation of spinophilin levels by imageJ revealed reduction in AD brain synaptosomes only in Braak stage 6 (Br-6) by 48% compared to age-matched controls. (c) Quantitation of levels of RanBP9-FL also showed alteration only in Br-6 which increased by 64%. (d) Quantitation of RanBP9-N60 also showed increase to 287% compared to age-matched controls. ANOVA followed by post hoc Tukey's test revealed significant differences. *P<0.05, **P<0.01 ***P<0.001 in Br-6 stage of AD synaptosomes compared to age-matched controls. The data are mean±S.E.M. and n numbers for each are indicated on the blots