Skip to main content
. Author manuscript; available in PMC: 2013 Jul 2.
Published in final edited form as: Nat Commun. 2013;4:1881. doi: 10.1038/ncomms2898

Figure 3. The effect of vitamin C is dependent on iron concentration.

Figure 3

(a) The iron scavenger deferoxamine (DFO) inhibits the bactericidal activity of vitamin C (VC) against M. tuberculosis H37Rv. M. tuberculosis H37Rv cultures were grown to an OD600nm of ≈0.7, diluted 1/20 and treated with with DFO (100 mg/L, 152 µM) and with or without 4 mM vitamin C. Growth was followed by plating for CFUs over time. (b) The growth of M. tuberculosis in Roisin medium with and without additional ferric ion (Fe, ferric ammonium citrate (0.04 g/l)) and treated or not with 4 mM vitamin C was followed by plating for CFUs over time. The experiments were done in triplicate and the average with standard deviation is plotted.