Table 2.
Transcriptional profile of vitamin C-treated M. tuberculosis reveals a possible displacement of divalent cations.
| Up-regulated | Down-regulated | ||||||
|---|---|---|---|---|---|---|---|
| Gene | Annotation | Cl | FC | Gene | Annotation | Cl | FC |
| psd | Phosphatidylserine decarboxylase | 1 | 3.11 | mbtD | Polyketide synthetase | 1 | −2.30 |
| dapE | Succinyl-diaminopimelate desuccinylase | 7 | 2.54 | fadA6 | Acetyl-CoA acetyltransferase | 1 | −2.30 |
| hsdM | Type I restriction system DNA methylase | 2 | 2.01 | Rv1096 | Possible glycosyl hydrolase | 7 | −2.10 |
| lytB1 | Probable Lytb-related protein | 3 | 1.98 | fbiC | F420 synthase | 7 | −2.06 |
| Rv1885c | Chorismate mutase | 7 | 1.91 | nei | Probable endonuclease VIII | 7 | −2.04 |
| Rv3833 | Transcriptional regulatory protein | 7 | 1.61 | umaA | Possible mycolic acid synthase | 1 | −1.99 |
| Rv0561c | Possible oxidoreductase | 7 | 1.57 | Rv2859c | Possible amidotransferase | 7 | −1.93 |
| accD2 | Probable acetyl-/propionyl-CoA carboxylase | 1 | 1.55 | pmmB | Probable phosphomannomutase | 7 | −1.83 |
| cysS | Cysteinyl-tRNA synthetase | 2 | 1.54 | glgP | Probable glycogen phosphorylase | 7 | −1.62 |
| Rv1167c | Probable transcriptional regulatory protein | 9 | 1.53 | glbN | Probable hemoglobin | 7 | −1.56 |
| lipP | Probable esterase/lipase | 7 | 1.42 | carA | Carbamoyl-phosphate synthase small subunit | 7 | −1.53 |
| nadD | Nicotinic acid mononucleotide adenyltransferase | 7 | 1.41 | hab | Probable hydroxylaminobenzene mutase | 7 | −1.45 |
| ureC | Urease alpha subunit (urea amidohydrolase) | 7 | 1.41 | mce4A | Mce-family protein | 0 | −1.39 |
| rpfA | Possible resuscitation-promoting factor | 3 | 1.38 | ahpD | Alkyl hydroperoxide reductase | 0 | −1.39 |
| Rv0892 | Probable monooxygenase | 7 | 1.32 | Rv1526c | Probable glycosyltransferase | 7 | −1.34 |
| ksgA | Dimethyladenosine transferase | 2 | 1.29 | rpmG2 | 50s ribosomal protein l33 | 2 | −1.27 |
| Rv2296 | Haloalkane dehalogenase | 7 | 1.26 | Rv3742c | Possible oxidoreductase | 7 | −1.25 |
| phoH1 | Probable PhoH-like protein | 7 | 1.24 | ephF | Possible epoxide hydrolase | 0 | −1.23 |
| argC | N-acetyl-gamma-glutamyl-phosphate reductase | 7 | 1.23 | rsfA | Anti-anti-sigma factor | 2 | −1.23 |
| cysK2 | Possible cysteine synthase A | 7 | 1.15 | lpqU | Probable conserved lipoprotein | 3 | −1.19 |
| ureD | Probable urease accessory protein | 7 | 1.15 | Rv0338c | Probable iron-sulfur-binding reductase | 7 | −1.19 |
| cobM | Probable precorrin-4 C11-methyltransferase | 7 | 1.14 | Rv3777 | Probable oxidoreductase | 7 | −1.11 |
| fadE16 | Possible acyl-CoA dehydrogenase | 1 | 1.13 | ideR | Iron-dependent repressor and activator | 9 | −1.10 |
| mce3C | Mce-family protein | 0 | 1.13 | prrA | Two-component response transcriptional regulatory | 9 | −1.10 |
| bioB | Biotin synthase | 7 | 1.07 | cysS | Cysteinyl-tRNA synthetase | 7 | −1.09 |
| dipZ | cytochrome biogenesis protein | 7 | 1.07 | moaE1 | Molybdenum cofactor biosynthesis protein | 7 | −1.09 |
| arsC | Arsenic-transport integral membrane protein | 3 | 1.07 | Rv2957 | Possible glycosyl transferase | 7 | −1.06 |
| Rv1516c | Probable sugar transferase | 7 | 1.06 | Rv0338c | Probable iron-sulfur-binding reductase | 7 | −1.19 |
| fixA | Probable electron transfer flavoprotein | 7 | 1.06 | Rv3777 | Probable oxidoreductase | 7 | −1.11 |
| cyp141 | Probable cytochrome p450 141 | 7 | 1.04 | ideR | Iron-dependent repressor and activator | 9 | −1.10 |
| grcC1 | Probable polyprenyl-diphosphate synthase | 1 | 1.03 | prrA | Two-component response transcriptional regulatory | 9 | −1.10 |
| folD | methylenetetrahydrofolate dehydrogenase | 7 | 1.03 | cysS | Cysteinyl-tRNA synthetase | 7 | −1.09 |
| Rv1158c | Conserved hypothetical ALA-, PRO-rich protein | 7 | 1.01 | moaE1 | Molybdenum cofactor biosynthesis protein | 7 | −1.09 |
| hemZ | Ferrochelatase | 7 | 0.98 | Rv2957 | Possible glycosyl transferase | 7 | −1.06 |
| cyp128 | Probable cytochrome P450 128 | 7 | 0.98 | ||||
| MT2963 | Siderophore utilization protein | 7 | 0.98 | ||||
| Rv1344 | Acyl carrier protein | 1 | 0.98 | ||||
| impA | Probable inositol-monophosphatase | 7 | 0.97 | ||||
| echA8 | Enoyl-CoA hydratase | 1 | 0.97 | ||||
FC: log2 Fold Change
Cl: Classification, based on the Tuberculist website (http://genolist.pasteur.fr/TubercuList/), stands for: 0, virulence, detoxification, adaptation; 1, lipid metabolism; 2, information pathways; 3, cell wall and cell processes; 7, intermediary metabolism and respiration; 9, regulatory proteins. The genes highlighted in bold are encoding enzymes containing divalent cations in their active sites. The experiment was done in triplicate.