FIG. 1.
Osmotic equilibrium plots for cells in phosphate buffered saline. (A) TF-1 cells, (B) HUVECs, (C) porcine chondrocytes, (D) human hepatocytes, (E) porcine hepatocytes, and (F) mouse oocytes. The symbols represent the experimental relative equilibrium cell volumes (V/Vo) in hypertonic solutions as a function of osmolality (π) (mean±SEM). The dashed line is the Boyle van't Hoff equation and represents the linear regression fit of Equation 7 to the data. The solid line represents the nonlinear regression fit of Equation 11 to the data. The y-intercepts (mean±95 % confidence intervals) give the ideal osmotically-inactive fraction, b, and the non-ideal osmotically-inactive fraction, b*. The respective adjusted R2 values are also shown. In the modified Boyle van't Hoff equation, inverse relative molality is the independent variable; however we have included the modified Boyle van't Hoff on the traditional Boyle van't Hoff graph for direct comparison.