Figure 5.

BHA blocks the occurrence of TAMs and suppresses tumorigenesis in Urethane-induced tumor model. (A, B) A/J mice were maintained on normal or BHA diet for 2 weeks prior to Urethane injection (A) or mice on normal or BHA diet 1 week after Urethane treatment (B). Lungs were collected 6 months after Urethane treatment, sectioned and immunostained for F4/80. Representative image is shown in the left panel (T, tumor area) and quantitative analysis is shown in the right panel. The F4/80⁺ cells were evaluated by counting 10 high power fields (20×) per lung section (1 mm)/two tissue sections/mouse. n = 10 mice. (C) Lung tissues from A were analyzed by western blotting with anti-arginase I, anti-YM-1 and anti-actin antibodies. (D) Representative H&E images of lungs of mice from A. (E) Lung tumor multiplicity of mice from (A) was determined by counting total tumor foci in 5 serial sections at 400 mm intervals. (F) Representative H&E images of lungs of mice from B. (G) Lung tumor multiplicity of mice from (B) was determined by counting total tumor foci in five serial sections at 400 mm intervals. Error Bars: ± SEM.