TABLE 2.
Antibodies Used in Immunohistochemistrya
| Antibody (Dilution) | Monoclonal isotype | Pre-treatment | Specificity | Source/reference |
|---|---|---|---|---|
| 7D4 (1:10) | IgM | K | Antibody recognizes a unique, as yet unidentified, native CS/DS sulfation motif epitope occurring towards the linkage region of GAG chain | Caterson (2012) |
| CS-56 (1:200) | IgM | K | Antibody reacts with an oligosaccharide sequence within generic CS, but not DS, chains. | Sigma Aldrich; Avnur and Geiger (1984); Ito et al. (2005) |
| 1B5 (neat) | IgG1 kappa | ABC/K | Antibody recognizes a chondroitinase ABC -generated 0-sulfated CS ‘stub'. | Couchman et al. (1984) |
| Caterson et al. (1985) | ||||
| Caterson (2012) | ||||
| 2B6 (neat) | IgG1 kappa | ABC/K | Antibody recognizes chondroitinase ABC- | Couchman et al. (1984) |
| generated -4 sulfated CS/DS ‘stubs'. | Caterson et al. (1985) | |||
| Caterson (2012) | ||||
| 3B3+ (1:10) | IgM,kappa | ABC/K | Antibody recognizes a chondroitinase ABC-generated -6 sulfated CS ‘stub'. | Caterson et al. (1985) |
| Caterson (2012) | ||||
| 5D4 (1:20) | IgG1 kappa | ABC | Antibody recognizes a linear oversulfated oligosaccharide within native KS chains. Identifies both type I (corneal) and type II (skeletal) KS forms. | Caterson et al. (1983) |
| 3G10 (1:100) | IgG2b | H/K | Antibody reacts with the unsaturated hexuronate (glucoronate) present at the non-reducing terminus of all heparitinase-generated HS ‘stubs'. | AMS biotechnology; David et al. (1992) |
a
ABC, chondroitinase ABC; CS, chondroitin sulfate; DS, dermatan sulfate; HS, heparan sulfate; K, proteinase K; KS, keratan sulfate; M, monoclonal; H, heparitinase.