Table I.
Flow cytometric analysis of splenic cell populations at 6 and 12 months of age.
| Cell population | FACS staining | 6 months | 12 months | ||||||
|---|---|---|---|---|---|---|---|---|---|
|
Sle16
No= 7 |
Sle16Sle18
No= 8 |
Sle18
No= 8 |
B6
No= 7 |
Sle16
No= 14 |
Sle16Sle18
No= 12 |
Sle18
No= 8 |
B6
No= 11 |
||
| Cells (106 /ml) | 62.18 ± 4.47$ | 38.49 ± 5.67 | 39.99 ± 5.02 | 43.09 ± 3.13 | 92.57 ± 6.99***, $$, † | 52.33 ± 2.77 | 50 ± 2.93 | 38.94 ± 4.77 | |
| % B cells (No ×106) |
CD19+ | 44.97 ± 2.2 (27.34 ± 2.48$) |
44.15 ± 1.36 (16.99 ± 2.54) |
45.92 ± 2.46 (18.29 ± 2.23) |
37.49 ± 1.65 (16.37 ± 1.76) |
42.29 ± 1.18*, $$$ (38.8 ± 2.73***, †) |
54.46 ± 1.18 (28.94 ± 1.95) |
46.97 ± 2.01 (23.5 ± 1.75) |
50.28 ± 2.01 (19.58 ± 2.56) |
| % T cells (No ×106) |
CD19−, CD5+ | 40.09 ± 1.57 (25.49 ± 1.85) |
43.87 ± 1.33 (16.96 ± 2.69) |
42.16 ± 2.76 (17.14 ± 2.91) |
45.72 ± 2 (19.62 ± 1.46) |
31.71 ± 1.48 (29.33 ± 2.71**, $$, †) |
28.94 ± 1.69* (15.34 ± 1.23) |
29.67 ± 1.71 (14.89 ± 1.25) |
36.22 ± 1.85 (13.96 ± 1.77) |
| % Plasma cells (No ×106) |
CD19−, CD90−,CD138+ | 0.69 ± 0.26 (0.42 ± 0.16) |
0.56 ± 0.11 (0.23 ± 0.05) |
0.56 ± 0.09 (0.2 ± 0.02) |
0.55 ± 0.03 (0.23 ± 0.01) |
2.05 ± 0.19***, ††† (1.94 ± 0.28***, $, †††) |
1.35 ± 0.11* (0.75 ± 0.09**) |
0.72 ± 0.07 (0.36 ± 0.04) |
0.62 ± 0.09 (0.21 ± 0.02) |
| % MZ B cells (No ×106) |
CD19gatedCD21highCD23low | 8.9 ± 1.64 (2.61 ± 0.55) |
11.02 ± 1.18 (1.99 ± 0.46) |
11.99 ± 1.2 (2.12 ± 0.28) |
9.2 ± 0.45 (1.51 ± 0.20) |
6.25 ± 0.69**, $ (2.4 ± 0.32) |
9.11 ± 0.56 (2.66 ± 0.28) |
6.87 ± 0.68* (1.59 ± 0.16) |
10.78 ± 0.82 (2.21 ± 0.38) |
| % FZ B cells (No ×106) |
CD19gatedCD21lowCD23high | 63.24 ± 3.73 (17.33 ± 2.68) |
63.13 ± 2.42* (10.45 ± 1.28) |
62.5 ± 1.18* (11.55 ± 1.57) |
73.43 ± 0.84 (12.09 ± 1.39) |
69.27 ± 1.37* (26.81 ± 1.86***, ††) |
67.13 ± 1.74 (19.13 ± 1.08) |
68.09 ± 3.79 (16.13 ± 1.8) |
61.4 ± 1.26 (11.95 ± 1.54) |
| % B1a cells (No ×106) |
CD19gatedCD5+ | 3.42 ± 0.25 (0.91 ± 0.06$) |
2.78 ± 0.25 (0.55 ± 0.06) |
3.76 ± 0.28 (0.65 ± 0.05) |
3.67 ± 0.16 (0.59 ± 0.05) |
2.89 ± 0.17$$ (1.11± 0.1*) |
3.98 ± 0.19 (1.19 ± 0.13*) |
3.84 ± 0.3 (0.9 ± 0.08) |
3.54 ± 0.29 (0.66 ± 0.08) |
| % activated B cells (No ×106) |
CD19gatedCD69+ | 10.22 ± 0.88 (2.85 ± 0.19$, ††) |
7.96 ± 0.41 (1.36 ± 0.21) |
6.95 ± 0.99 (1.22 ± 0.23) |
9.14 ± 0.74 (1.44 ± 0.10) |
16.61 ± 0.93***, ††† (6.42 ± 0.61***, †††) |
13.78 ± 0.77*, ‡‡ (4.02 ± 0.35*, ‡) |
8.61 ± 0.31 (2 ± 0.12) |
9.33 ± 0.52 (1.86 ± 0.27) |
| % activated T cells (No ×106) |
CD4gatedCD69+ | 20.56 ± 2.6** (5.86 ± 0.68*) |
19.29 ± 0.87* (3.18 ± 0.41) |
18.69 ± 0.97 (3.21 ± 0.58) |
12.89 ± 0.78 (2.55 ± 0.28) |
34.54 ± 1.41** (10.22 ± 1.11***, $, ††) |
35.39 ± 1.8*** (5.5 ± 0.58) |
26.34 ± 2.72 (3.79 ± 0.34) |
22.65 ± 1.27 (3.14 ± 0.41) |
| % naïve T cells (No ×106) |
CD4gatedCD62L+ | 57.84 ± 3.26 (14.6 ± 0.98) |
60.37 ± 1.46 (10.55 ± 2.16) |
63.37 ± 1.77 (11.11 ± 2.14) |
65.52 ± 1.61 (12.94 ± 1.14) |
¶28.76 ± 2.23**, †† (8.27 ± 1.17) |
41.27 ± 2.43 (6.4 ± 0.65) |
47.57 ± 3.6 (7.2 ± 0.96) |
50.03 ± 2.03 (7.2 ± 1.08) |
| % Memory T cells (No ×106) |
CD4gatedCD44highCD25− | 28.39 ± 2.5 (7.43 ± 1.11†) |
28.51 ± 1.49 (4.59 ± 0.53) |
25.74 ± 1.9 (4.11 ± 0.44) |
24.04 ± 1.02 (4.67 ± 0.31) |
50.19 ± 1.78*** (14.66 ± 1.4***, $, ††) |
48.46 ± 1.86*** (7.33 ± 0.58) |
41.64 ± 3.3 (6.01 ± 0.45) |
33.4 ± 1.49 (4.46 ± 0.43) |
| % Regulatory T cells (No ×106) |
CD4gatedCD25+FoxP3+ | 20.47 ± 1.95** (5.24 ± 0.7**) |
17.61 ± 0.97** (2.98 ± 0.4) |
16.88 ± 0.81* (2.8 ± 0.37) |
10.17 ± 0.3 (2.01 ± 0.2) |
27.83 ± 1.51* (8.18 ± 0.92***, $) |
27.62 ± 1.11* (4.29 ± 0.43) |
Not available | 22.59 ± 1.23 (3.03 ± 0.32) |
Results are shown as mean ± SEM. P values considered significant when <0.05 according to the Kruskal-Wallis test with Dunn’s multiple comparison test. Statistical analysis as follow:
*
any congenic vs B6,
$
Sle16 vs Sle16Sle18,
†
Sle16 vs Sle18;
‡
Sle16Sle18 vs Sle18.
One symbol: <0.05, two symbols: <0.01, three symbols: <0.001.
¶
CD62L staining was available only for 6 mice. When gated cells are indicated, the percentage refers to the percentage of the gated population. No = number; MZ= marginal zone; FZ: follicular zone.