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. 2013 Jun 21;2(3):e000134. doi: 10.1161/JAHA.113.000134

Figure 4.

Figure 4.

Expression of TDAG51 in atherosclerotic lesions. Six‐week‐old TDAG51+/+/ApoE−/− and TDAG51−/−/ApoE−/− mice were fed control chow diet for 25 weeks. Mice were euthanized, and hearts containing aortic roots were removed, embedded in paraffin, sectioned, and immunostained for TDAG51. Indirect immunofluorescence detection of TDAG51 in lesions (arrows) from aortic roots of TDAG51+/+/ApoE−/− mice (A and B). B, Magnified region from A. Representative images from 5 mice are shown. To assess nonspecific immunofluorescence, aortic root sections from TDAG51−/−/ApoE−/− mice were immunostained using an anti‐TDAG51 antibody (C). Scale bars: 100 μm in A and C; 50 μm in B. TDAG51 indicates T‐cell death‐associated gene 51; ApoE, apolipoprotein E.