Figure 4.

Expression of costimulatory molecules on smooth muscle cells (SMCs). Histogram depicting fluorescent staining of SMCs for B7‐1 (A, top) and B7‐2 (A, bottom). Representative of 3. B7‐1 antibody is allophycocyanin (APC) conjugated and B7‐2 antibody is Alexa Fluor 700 conjugated. Reverse‐transcription polymerase chain reaction (RT‐PCR) of injured carotid arteries from wild‐type (WT) mice for B7‐1 mRNA expression (B, top) with β‐actin as reference gene. Bar graph of densitometric analysis of signal intensity relative to reference gene (B, bottom). Right carotid arteries pooled from 4 to 5 mice per time‐point.