Figure 4.

Chronic angptl2 infusion for 1 month ATX mice accelerates atherogenesis in 3‐month‐old ATX mice. A, Infusion with angptl2 promotes the expression of inflammatory cytokines and adhesion molecules in freshly scraped ECs from the aorta evaluated by quantitative RT‐PCR and normalized by cyclophilin A. B, angptl2 accelerates the formation of atherosclerotic plaque. C, angptl2 increases total cholesterol and LDL plasma levels. Data are mean±SEM of n=5 angptl2‐infused mice and n=5 TBSE‐infused mice. *P<0.05 vs TBSE condition. Angptl2 indicates angiopoietin like‐2; EC, endothelial cell; WT, wild type; RT‐PCR, real‐time polymerase chain reaction; LDL, low‐density lipoprotein; TBSE, Tris‐base, Sodium chloride, EDTA.