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. 2013 Jun 21;2(3):e004796. doi: 10.1161/JAHA.113.004796

Figure 8.

Figure 8.

Metabolic remodeling and mTOR activation precede structural remodeling in hearts subjected to high workload in vivo. A, Representative serial transverse, end‐diastolic PET slices for TAC and sham‐operated mice 1 day, 2 weeks, and 4 weeks after surgery. One day after TAC, there was an increase in FDG uptake that increased further over 4 weeks. B, Quantification of the rate of cardiac FDG uptake (Ki) of PET images from all TAC (n=8) and sham‐operated (n=5) mice. Data shown are mean±SEM. Ki in TAC mice demonstrated a 5‐fold increase in FDG uptake on day 1 and a 1.5‐ to 3.2‐fold increase from day 1 to 4 weeks. Sham‐operated mice showed no significant change in FDG uptake over 4 weeks. Comparisons at different times within TAC group: day 1 vs baseline (BSL), #P<0.05; 2 weeks vs BSL or day 1, **P<0.05; 4 weeks vs BSL, day 1, or 2 weeks, *P<0.001. Comparisons between TAC and sham groups at the same points, ^P<0.05. C, Tissue G6P levels in hearts after TAC or sham operation at baseline and after 1 day and 2 weeks. Dot plots of G6P levels for each group; n=6 for TAC and n=5 for sham. G6P levels were 2.3‐ and 4.6‐fold higher compared with sham‐operated animals 1 day and 2 weeks after TAC, respectively. Kruskal–Wallis test yielded overall P=0.0356. D, Representative Western blots demonstrated an increase in p70S6K and 4EBP1 phosphorylation 1 day and 2 weeks after TAC. mTOR indicates mammalian target of rapamycin; PET, positron emission tomography; TAC, transverse aortic constriction; FDG, 2‐deoxy, 2[18F]fluorodeoxy‐glucose.