Relationship between dietary eicosapentaenoic acid (EPA) plus docosahexaenoic acid (DHA) consumption and plasma phospholipid EPA+DHA concentrations among 2,692 older US adults, evaluated using restricted cubic splines, and adjusted for age, sex, race, and education. Because the dietary questionnaire estimated only EPA+DHA (and not DPA), for comparability we evaluated circulating EPA+DHA (rather than EPA+DPA+DHA). Median circulating levels of EPA+DHA in the highest quintile were ~5 percent of total fatty acids. The solid line and shaded area represent the central estimate and 95% CIs, respectively. There was strong evidence for both an overall trend (P<0.001) and nonlinearity of this relationship (P<0.001).