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. Author manuscript; available in PMC: 2014 Jun 7.
Published in final edited form as: Neurosci Lett. 2013 Apr 17;544:152–156. doi: 10.1016/j.neulet.2013.04.004

Figure 1.

Figure 1

Purkinje cell-specific deletion of p38. Pcp2-Cre mice were cross-mated with floxed-p38α mice. Animal tail samples and PCR method were used to identify genotypes. Figure 1A illustrates the DNA images of Pcp2-Cre, homozygous p38loxP/loxP, and an internal positive control (DNA quality control) at 100, 414, and 324 base pair (bp), respectively. In Figure 1B, the cerebellar sections of these mice were immunostained using p38α antibody. Wild-type mice (C57BL/6 mice) and transgenic mice (Pcp2-Cre-/-/p38loxP/loxP) without Pcp2-Cre (Figure 1A-b) show p38 positive stains (dark brown deposits) in the Purkinje neurons along the Purkinje layer. The p38 markers do not appear in transgenic mice that express both Pcp2-Cre and p38loxP/loxP(Pcp2-Cre+/-/p38loxP/loxP) (Figure 1A-a).