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β-galactosidase expression in aging hCB-ECs as measured by the fluorescence of the substrate, FDG. FDG intensity was normalized to the DAPI fluorescence per well. β-galactosidase levels increased with increased population doublings. For each experiment, 3 replicates were performed (n=3) *:p<0.05 compared to 18 or 22 population doublings; #:p<0.01 compared to 18 or 22 population doublings.
