Fig. 2.
Short and long-term effects of a single H2O2 treatment on endothelial permeability to albumin. hCB-ECs and HAECs that underwent fewer than 31 population doublings were incubated with 200 μM H2O2 for 3 hours and albumin permeability was measured over a two hour period either immediately after or 24 hours after H2O2 treatment. Immediately after treatment hCB-EC and HAEC permeability to albumin was elevated. 24 hours after treatment, the HAECs still had an elevated permeability and the hCB-EC permeability was similar to that of the untreated control. For each experiment, 8 replicates were performed (n=3–5) *:p<0.05 compared to untreated control.