Table 1.
Genome Assembly Statistics
| Spiroplasma chrysopicola DF-1 | S. syrphidicola EA-1 | S. melliferum IPMB4A | S. melliferum KC3 | S. citri GII3-3X | |
|---|---|---|---|---|---|
| GenBank accession | CP005077 | CP005078 | AMGI01000001– AMGI01000024 | AGBZ01000001– AGBZ01000004 | AM285301– AM285339 |
| Number of chromosomal contig(s) | 1 | 1 | 24 | 4 | 39 |
| Combined size of chromosomal contig(s) (bp) | 1,123,322 | 1,107,344 | 1,098,846 | 1,260,174 | 1,525,756 |
| Estimated chromosomal size (bp) | — | — | 1,380,000 | 1,430,000 | 1,820,000 |
| Estimated coverage (%) | — | — | 79.6 | 88.1 | 83.8 |
| G + C content (%) | 28.8 | 29.2 | 27.5 | 27.0 | 25.9 |
| Coding density (%) | 89.0 | 90.4 | 85.1 | 83.0 | 80.2 |
| Protein-coding genesa | 1,009 | 1,006 | 932 | 1,222 | 1,905 |
| Length distribution (Q1/Q2/Q3)(a.a.) | 171/278/425 | 176/279/425 | 176/280/440 | 119/233/376 | 83/149/286 |
| Plectrovirus proteinsb | 0 | 0 | 11 | 132 | 375 |
| Hypothetical proteins | 394 | 410 | 337 | 485 | 519 |
| Annotated pseudogenesa | 6 | 3 | 12 | 12 | 401 |
| rRNA operon | 1 | 1 | 1 | 1 | 1 |
| tRNA | 33 | 32 | 32 | 31 | 32 |
| Number of plasmids | 0 | 0 | 0 | 4 | 7 |
aFor S. chrysopicola, S. syrphidicola, and S. melliferum IPMB4A, putative pseudogenes were annotated with the “/pseudo” tag in gene feature as suggested by the NCBI GenBank guidelines and were not counted in the total number of protein-coding genes. For S. melliferum KC3 and S. citri, putative pseudogenes were annotated by adding the term “truncated” in the CDS product description field and were included in the total number of protein-coding genes.
bMost of the plectrovirus-related regions were excluded from the final S. melliferum IPMB4A assembly due to unresolvable polymorphism, resulting in a lower number of plectroviral genes (Lo et al. 2013).