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CD11c+ DC isolated from pooled lymph nodes of FLT3L-treated mice were cultured in medium alone or with CpGA (5μg/ml) in the presence of Wnt3A (2μg/ml) or Wnt5A (2μg/ml). Cells were analyzed by FACS at 6 hours of culture. Expression of CCR7, MHCII, CD80 and CD86 was determined of conventional DC, gated as CD11c+ Siglec H- cells. Data are from one of two experiments performed with similar results.
