Figure 3.

Trypanosoma brucei treated with 17-AAG are more sensitive to heat shock. Starting at −1.5 hours, cells at 37°C were treated with 17-AAG or solvent as indicated. The temperature for control cells remained at 37°C for the duration of the experiment. At −1 hour, tubes containing cells to be heat shocked (HS) were transferred to a 41°C water bath and then returned to a 37°C environment at 0 hours for the remainder of the experiment. Motile cells were counted by hemocytometer to determine parasite density. Values are the mean ± SD of 3 determinations.