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. 2013 Jul 15;140(14):3018–3027. doi: 10.1242/dev.088336

Fig. 5.

Fig. 5.

Notch-related phenotypes in Catsup-deficient tissues and cells. (A) Wild-type adult Drosophila wing blade. (B) Adult wing bearing a Catsup47 mutant clone, showing wing notching and missing wing margin material at tip. (C-F) Representative adult wing notching phenotypes of Notch54l9/+ heterozygotes (C), Notch54l9/+; Catsup26/+ double heterozygotes (D), Notch264-107/+ heterozygotes (E) and Notch264-107/+; Catsup26/+ double heterozygotes (F). (G) Average number of notches per wing blade in Notch54l9/+ and Notch264-107/+ heterozygotes in combination with either a Catsup wild-type genotype (WT), Catsup47/+ (47), Catsup48/+ (48) and Catsup26/+ (26), as denoted at the bottom; asterisks indicate Catsup heterozygous mutant genotypes that exhibit statistically significant (P<0.05) increases in wing notching incidence relative to Catsup+ (n=50 flies/100 wings per genotype. Notch54l9 genotypes: wild type=0.20±0.049; Catsup47=0.79±0.081, P=2.6×10-9; Catsup48=0.34±0.064, P=0.084; Catsup26=1.23±0.066, P=2.1×10-20. Notch264-107 genotypes: wild type=0.90±0.075; Catsup47=2.01±0.073 P=5.9×10-23; Catsup48=1.99±0.086, P=4.0×10-18; Catsup26=2.26±0.090, P=2.6×10-24). The Catsup wild-type genotype used for these crosses was the parental stock y w; P{ry[+t7.2]=neoFRT}40A P{w[+mW.hs]=FRT(whs)}G13 employed in our initial mutagenesis screen. Data are mean numbers of notches per wing ± s.e.m. (H-I′) Expression of the Notch target Cut (red in H′,I′) in p35-expressing wild-type control clones (H-H′) and Catsup mutant clones (I-I′), in which the clone cells are positively marked by p35 expression (green in H and I). Merged images of the Cut and p35 signals are shown on the right in H′ and I′; white arrowhead in I and I′ indicates Catsup mutant cells at the DV boundary that fail to express Cut. (J-J′) Overexpression of Catsup-V5 (green) under the control of patched-GAL4 (J) does not affect Cut expression (J′; red) where Catsup-V5 overexpression intersects with the DV boundary; merged signals for Catsup-V5 and Cut expression are shown in J′.