Fig. 3.

Determining species-specific percentages of cells. (A) Chick- and quail-specific primers were used with a dilution series of cDNA from both chick (green squares) and quail (red squares). Linear regressions were calculated (solid line), as were confidence intervals (dashed lines). Values for Chimera #1 and Chimera #2 (pink circles) were calculated from the regression lines. (B,C) Percentages calculated for Chimera #1 and Chimera #2 with RPL19 were validated using FACS to count GFP-positive chick cells.