Figure 4.

Valproic acid downregulates transcription of cyclin D1, cyclin E1, CDK4, and CDK6, and upregulates transcription and protein expression of p21. Comparison between control group and valproic acid after two weeks of treatment. (A) Expression of cyclin D1, cyclin E1, CDK4, and CDK6 detected by reverse transcriptase polymerase chain reaction. (B) Expression of cyclin D1, E2F, and retinoblastoma protein by Western blotting.

Abbreviations: Con, control group; VPA, valproic acid group; CDK, cyclin-dependent kinase.