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Valproic acid upregulates p21 expression by enhancing acetylation of histone H3 in p21 promoter region. Comparison between control group and valproic acid group after two weeks of treatment. (A) Detection of p21 expression by reverse transcriptase polymerase chain reaction. (B) Detection of p21 expression by Western blotting. (C) Detection of histone H3 acetylation level in p21 promoter by chromatin immunoprecipitation.
Abbreviations: Con, control group; VPA, valproic acid group.
