Fig. 6.

Phosphorylated cAMP response element-binding protein (pCREB) in striatal neuropeptide Y (NPY) interneurons following in vivo WIN 55,212-2 administration. (A–D) Striatal sections from 12-week-old R6/2 and wild-type littermates dual-labeled for pCREB (green) and NPY (Blazquez et al., 2011) 30 min following i.p. administration of WIN 55,212-2 or vehicle (scale bar: 100 µm). Enlarged images of NPY interneurons depicting the presence or absence of nuclear pCREB staining (left three panels of A–D; scale bar: 10 µm). (E) sq-IHC of pCREB increases in striatal NPY interneurons of 12-week-old R6/2 and wild-type littermates 30 min following treatment with WIN 55,212-2 or vehicle (n = 5 mice; ***P < 0.001). All values are expressed as mean ± SEM normalized to age-matched wild-type pCREB expression, and analysed by a one-way anova with Tukey’s post hoc test.