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. Author manuscript; available in PMC: 2013 Jul 2.
Published in final edited form as: Nat Chem Biol. 2012 Oct;8(10):862–869. doi: 10.1038/nchembio.1064

Figure 4. Transition into the hexagonal phase state induces acyl specificity.

Figure 4

Incubations contained purified tafazzin, CL (various molecular species), and 19:0-LPC (CL:LPC=9:1). Mixtures of two CL species were supplied at a molar ratio of 1:1. CaCl2 was added at a concentration of 20 mM in order to convert lipids to the hexagonal phase. (a) Mass spectra demonstrate the composition of PC species produced by transacylation. In the presence of Ca2+, the transacylation becomes 18:2-specific. (b) Bar graphs show the ratios of PC species formed by the indicated pairs of CL species in the absence and presence of Ca2+. The data document that addition of Ca2+ alters acyl specificity.