Table 2. Parameters of PL/LPL mixtures determined by magic angle spinning 31P NMR spectroscopy.
| PL/LPL mixturea (mol : mol) |
Isotropic chemical shift (ppm) |
Relative peak area (%) |
CSA (ppm) |
Line width (Hz) |
Phase characteristics |
|---|---|---|---|---|---|
| PC | −1.28 | 100 | 18 | 679 | Lamellar |
| PC/LPC (8:2) | −5.57 | 21 | 2.7 | 1387 | Lamellar |
| −0.70 | 79 | 17 | 497 | ||
| PC/LPC (2:8) | −0.82 | 22 | 0 | 26 | Isotropic |
| −0.37 | 78 | 0 | 17 | ||
| PE | −0.12 | 100 | −9.9 | 63 | Hexagonal |
| PE/LPC (9:1) | −0.11 | 100 | −3.9 | 66 | Non-lamellar |
| PE/LPC (2:8) | −0.27 | 48 | 5.2 | 82 | Non-lamellar |
| 0.41 | 52 | −7.4 | 589 | ||
| CL | 0.40 | 100 | 17 | 131 | Lamellar |
| CL/LPC (9:1) | −1.79 | 22 | −11 | 300 | Lamellar + Non-lamellar |
| 0.07 | 78 | 7.9 | 340 | ||
| CL/LPC (8:2) | −0.27 | 7 | −4.9 | 129 | Lamellar + Non-lamellar |
| −0.11 | 63 | −9.8 | 745 | ||
| 0.29 | 30 | 15 | 153 | ||
| CL/LPC (2:8) | −0.29 | 59 | 0 | 29 | Isotropic |
| 0.20 | 41 | 0 | 41 | ||
| CL + Ca2+ | −2.35 | 100 | −16 | 104 | Hexagonal |
| CL/LPC (8:2) + Ca2+ | −2.16 | 100 | −13 | 125 | Hexagonal |
| CL/LPC (2:8) + Ca2+ | −0.91 | 67 | −0.05 | 174 | Isotropic |
| −0.50 | 33 | 0 | 35 |
a
lipids include 18:2-18:2-PC, 18:2-18:2-PE, bovine heart CL [predominantly (18:2)4-CL], and 14:0-LPC