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. 2013 Jun 23;13:285. doi: 10.1186/1471-2334-13-285

Figure 5.

Figure 5

R. conorii infection-initiated cytoplasmic translocation of exogenous rANG enhanced VE-cadeherin internalization into endothelial cells and tyrosine phosphorylation of VE-cadherin at 72 hrs p.i. Dual immunofluorescence staining of SFG rickettsiae (red) and VE-cadherin (green) in human umbilical vein endothelial cells (HUVECs) using a dual wave length filter system revealed that internalized VE-cadherin could be detected at 72 hrs p.i. (image E), compared to mock and 24 hrs p.i. (image A, C). Addition of rANG shows an enhancing effect on the internalization of VE-cadherins in HUVECs at 72 hrs p.i. (image F). A representative immunoprecipitation (IP) study suggested that exogenous rANG enhances phosphorylation of VE-cadherin (p-VE-cadherin) at 72 hrs p.i. (image G). Nuclei of HUVECs are counter-stained with DAPI (blue).