Figure 2.

Reduced total jejunal ACS activity in Acsl5 KO mice. Jejunal mucosa (A) and liver tissues (B) from female or male Acsl5 wild type (WT), heterozygous (Het) or knockout (KO) mice, 5 animals per group, were harvested and homogenized. Equal amounts of protein were incubated in the presence of raiolabeled palmitic acid, ATP and CoA. Unconjugated fatty acid was extracted in heptane and aqueous phase radioactivity, containing the water soluble palmitoyl-CoA was determined by scintillation counting. Mean±SD palmitoyl-CoA synthesis rates are presented.