Figure 5. Effects of zinc on arsenite-dependent increase of ssUVR-induced DNA damage in vivo.
SHK-1 mice were treated with arsenite (As, 5 mg/l), zinc (10, mg/l), both (As+Zn) or nothing (NT) in their drinking water for 28 days. Animals were then exposed to a single dose of ssUVR (UVR, 28 kJ/m2, 1.2 MED). Irradiated dorsal skin was collected after 1 h, formalin fixed, processed and sections stained as described in “Materials and Methods”. (A) Confocal microscopy images representative of CPD staining from animals treated as indicated with ratio of stained nuclei per total nuclei graphically represented in (C). (B) Images representative of sections peroxidase stained for pH2AX from animals treated as indicated. Arrows indicate examples of positive nuclei. Graphical representation of ratio of pH2AX (D) or 6,4-PP (E) stained nuclei per total nuclei. Bar = 10 mm. * = significantly different between indicated groups; p <0.05; n ≥ 5.