Figure 4.

Determination of sensitivity profile to the enzymes β-1,3-glucanase and zymolyase. (A) myo1p-METUBC4, myo1 (YJR13), myo1 ubc4, wt (YJR12) and wtpMETUBC4 cells were incubated with 120 U β-1,3-glucanase at 25 °C for up to 75 min, as described in Materials and methods. To obtain their sensitivity profiles, the OD_600nm ratio was determined by dividing the absorbance units at any time point by the absorbance units at t₀. Chart presents the average of three experiments. (B) The same strains were incubated with 5 μg/ml zymolyase at 37 °C for up to 4 h, as described in Materials and methods. The OD_600nm ratio was determined as decribed above. Chart presents the average data from three experiments