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. Author manuscript; available in PMC: 2013 Jul 2.
Published in final edited form as: Yeast. 2007 Apr;24(4):343–355. doi: 10.1002/yea.1481

Figure 5.

Figure 5

Assessment of the role of proteasome and PVC/LE pathways in acquired NZ resistance. (A) Effects of 10 μM β-lactone on NZ resistance of wt, myo1, myo1 cells overexpressing UBC4 (myo1pUBC4), myo1 ubc4 and wt cells overexpressing UBC4 (wtpUBC4). Strains with high NZ resistance show a low growth inhibition ratio and vice versa. The growth inhibition ratio was calculated as follows: OD600nm treated/OD600nm untreated. The chart represents the average of at least three experiments. (B) NZ resistance agar plate assays of the indicated strains were conducted by drop tests (from left to right, 107 – 102 cells/ml serial dilutions) on ura− gal agar plates (untreated) and ura− gal agar plates with 30 μg/ml NZ. NZ-resistant wild-type (wt) and myo1pUBC4 cells and sensitive myo1 cells were included as controls. The plates were incubated at 27 °C for 4 days before assessing growth and photographing