Table 1.
Identification of cDNAs whose overexpression confers NZ-resistance to myo1 cells
| Systematic name | Gene | Redundancy (No. of clones) | NZ resistance (%) | Description |
|---|---|---|---|---|
| YPL177C | CUP9 | 1 | 84.2 | Homeodomain-containing transcriptional repressor of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription |
| YGR175C | ERG1 | 1 | 81 | Squalene epoxidase, catalyses the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine |
| YMR043W | MCM1; FUN80 | 1 | 92.5 | Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes |
| YOL126C | MDH2 | 1 | 80 | Cytoplasmic malate dehydrogenase, one of the three isozymes that catalyse interconversion of malate and oxaloacetate; involved in gluconeogenesis during growth on ethanol or acetate as carbon source; interacts with Pck1p and Fbp1p |
| YOL064C | MET22 | 1 | 100 | Bisphosphate-3′-nucleotidase, involved in salt tolerance and methionine biogenesis; dephosphorylates 3′-phosphoadenosine-5′-phosphate and 3′-phosphoadenosine-5′-phosphosulfate, intermediates of the sulphate assimilation pathway |
| YDR505C | PSP1; GIN5 | 1 | 91.9 | Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase-α) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition |
| YDR382W | RPP2B; RPL45; YPA1 | 3 | 87.9 | Ribosomal protein P2-β, a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm |
| YHL015W | RPS20; URP2 | 1 | 93 | Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; has similarity to E. coli S10 and rat S20 ribosomal proteins |
| YER074W | RPS24A; RPS24EA | 1 | 89.6 | Protein component of the small (40S) ribosomal subunit; identical to Rps24Bp and has similarity to rat S24 ribosomal protein |
| YOR182C | RPS30B | 1 | 83.6 | Protein component of the small (40S) ribosomal subunit; nearly identical to Rps30Ap and has similarity to rat S30 ribosomal protein |
| YPL237W | SUI3 | 2 | 100 | β-subunit of the translation initiation factor eIF2, involved in the identification of the start codon; proposed to be involved in mRNA binding |
| YDR177W | UBC1 | 1 | 81.1 | Ubiquitin-conjugating enzyme that mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response |
| YBR082C | UBC4 | 4 | 87.9 | Ubiquitin-conjugating enzyme that mediates degradation of short-lived and abnormal proteins; interacts with E3-CaM in ubiquitinating calmodulin; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response |
| YDR495C | VPS3; PEP6; VPL3; VPT17 | 1 | 96.6 | Cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase |