Table 1.
Characteristics of the E. coli and S. cerevisiae strains and plasmids used in this study
| Strains and plasmids | Relevant features | Reference |
|---|---|---|
|
E. coli strain |
|
|
| Novablue |
endA1 hsdR17(rK12-mK12+) supE44 thi-I gyrA96 relA1 lac recA1/F’[proAB+lacIq Z∆M15::Tn10(Tetr)] |
Novagen |
|
S. cerevisiae yeast strains |
|
|
| MT8-1 |
MATa ade leu2 his3 ura3 trp1 |
Tajima et al. (1985) |
| MT8-1/cocδBEC1 |
MATa ade leu2 his3 ura3, Cocktail δ-integration of β-glucosidase, endoglucanase, and cellobiohydrolase genes |
Yamada et al. (2010b) |
| MT8-1/δCDTiBGL |
MATa ade leu2 his3 trp1, Cocktail δ-integration of intracellular β-glucosidase and cellodextrin transporter genes |
This study |
| MT8-1/cocδBEC1/δCDTiBGL |
MATa ade leu2, Cocktail δ-integration of β-glucosidase, endoglucanase, cellobiohydrolase, intracellular β-glucosidase, and cellodextrin transporter genes |
This study |
| Plasmids |
|
|
| pδU-PGCDT |
URA3, Expression of cellodextrin transporter by δ-integration |
This study |
| pδU-PGiBGL |
URA3, Expression of intracellular β-glucosidase by δ-integration |
This study |
| pδH-PGiBGL | HIS3, Expression of intracellular β-glucosidase by δ-integration | This study |