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. 2013 Jun 27;105(13):937–946. doi: 10.1093/jnci/djt120

Figure 3.

Figure 3.

MyD88 extinction, Erk-MAPK signaling, and caspase-dependent apoptosis. A) Apoptosis of HCT116 p53+/+ or p53–/– cell lines upon MyD88 silencing by small interfering RNA (siRNA) and treatment with either caspase inhibitor ZVAD or its control (ZFA). B) Western blot analysis of the expression of caspase 9, caspase 3, and PARP proteins in HCT116 p53+/+ or p53–/– cells upon MyD88 silencing by siRNA. C) Apoptosis analysis of HCT116 p53+/+ or p53–/– cells upon silencing of MyD88 and/or NF-κB p65. D) Apoptosis of HCT116 p53+/+ expressing a doxycycline-inducible MyD88 short hairpin RNA (shMyD88), transfected with either an empty vector or with constitutively activated MEK, after treatment or no treatment (NT) with doxycycline (DOX). Each experiment was performed three independent times, with each condition done in triplicate. Error bars indicate standard deviation. For statistical analysis, one-sided Student t test was used, with P values indicated in the figure. NS = not significant; siCtrl = non-silencing siRNA; siMyD88 = MyD88-specific siRNA.