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. 2013 Jun 27;105(13):937–946. doi: 10.1093/jnci/djt120

Figure 4.

Figure 4.

MyD88 silencing, ERCC1, and DNA damage. A) Western blot analysis of the expression of DNA repair enzyme ERCC1 in HCT116 p53+/+ or p53–/– cells upon MyD88 silencing by small interfering RNA (siRNA). B) Fluoroescence-activated cell sorting (FACS) analysis of DNA damage foci accumulation as revealed by the phosphorylation of the S139 of histone H2AX, in HCT116 p53+/+ or p53–/– cells upon MyD88 silencing by siRNA. C) Quantification of DNA damage foci accumulation (pS139 H2AX), by FACS analysis in HCT116 p53+/+ cells upon MyD88 silencing by siRNA, and transfection with either an empty vector, with a constitutively activated MEK, or with ERCC1. D) Apoptosis of HCT116 p53+/+ cells upon MyD88 silencing by siRNA, and transfection with either an empty vector, or with a constitutively activated MEK, or with ERCC1. Each experiment was performed three independent times, with each condition done in triplicate. Error bars indicate standard deviation. For statistical analysis, one-sided Student t test was used, with P values indicated in the figure. Ctrl = control; siCtrl = non-silencing siRNA; siMyD88 = MyD88-specific siRNA.