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. 2013 Jul 2;11(7):e1001597. doi: 10.1371/journal.pbio.1001597

Figure 2. The Erf2–Erf4 palmitoyltransferase drives major changes in the meiotic palmitoylome during meiosis.

Figure 2

(A–B) Alk-16-associated fluorescence of immunopurified Ras1 from vegetatively growing cells with the indicated palmitoyltransferase deletions (top panels). Western blots are probed for Ras1 (bottom panels). Wild-type (DHHC) or catalytically inactive (DHHA) Erf2 was expressed from a thiamine-repressible promoter in erf2Δ cells (B). (C) Fluorescence detection of palmitoylated substrates (top panel) and Ras1 palmitoylation (bottom panel) in homozygous diploid pat1-114/pat1-114 cells with the indicated palmitoyltransferase deletions 8 h after meiotic induction. Ctrl, DMSO control; Veg, vegetative cells.