Figure 6. Palmitoylation-dependent Rho3 function is required for the Erf2-induced meiotic phenotype.

(A) DAPI (top) and DIC (bottom) images of erf2 and erf4 co-overexpressing cells with indicated genotypes at 96 h postinduction. Vectors expressing internally tagged wild-type and mutant (Cys4→Ala) Rho3-HA₃ proteins from the nmt41 promoter were integrated into the chromosome of rho3Δ cells. Scale bars, 10 µm. (B) Alk-16-associated fluorescence of Rho3 and palmitoylation-deficient Rho3(C4A) (top panel). Western blots were probed for HA (bottom panel).